| 1. | The ha 1 gene was inserted into the bacterial plasmid pgex - 4t - 2 and the recombinant plasmids containing ha 1 gene were identified by restriction enzyme analysis and pcr mathod
结果表明同源性分别达到98和97 ，并且在ha1切割位点有多个碱性氨基酸的插入序列，证明其为强毒株。 |
| 2. | For the products of primary rt - pcr and nested - pcr are all encompassing the hyper - variable region of vp2 gene , so we can take a restriction enzyme analysis ( rea ) directly
第二，本研究的基础rt - pcr和nested - pcr所扩增的片段均是横跨ibdv的vvp2的，所以，可直接对pcr产物进行酶切分型研究。 |
| 3. | Proper multi - copy gene was selected and cloned into puc19 vector . restriction enzyme analysis and dna sequencing confirmed that 5 - copy gene was correctly inserted into the vector
选取合适拷贝数的串连重复基因，将其克隆至puc19载体，双酶切、 pcr扩增和dna测序证明串连重复基因构建成功且基因方向相同。 |
| 4. | We confirmed the correct construction by pcr and restriction enzyme analysis . in this research , hypocotyls were used as the explant and several factors affecting genetic transformation of carrot mediated by agrobacterium tumefaciens were studied
利用vp7基因和质粒pbi121上相同的单克隆位点，将vp7基因定向克隆到植物表达载体pbi121上，构建了pbi121vp7表达载体。 |
| 5. | Conclusion : by restriction enzyme secting , ligating , transforming , restriction enzyme analysis , and final dna sequencing , the pbd - i and pbd - ii gene were proved to be recombinated with the expression vector and the recombinated vector ppd - 1 and ppd2 were transformed successfully
结论：经过酶切、连结，构建成重组质粒ppd上、 ppd上，再经转化、抽提质粒及酶切分析，最后经dna测序证实， rticr扩增的pbd i 、 pbd 11基因与piflpdt ” xsi表达载体构建成功。 |
| 6. | When the patient with regional enteritis complains of the symptoms arising from disaccharidase dificiency , the clinician should be alert to evaluate an elimination diet starting with lactose containing components and consider diagnostic studies using tolerance tests and / or enzyme analyses of small bowel mucosa , if ( it is ) available
节段性肠炎病人诉述由于二醋缺乏所引起的症状时，临床医师就应机智地为病人计算一种开始时包含乳糖成分的脱敏食谱，并且考虑做一些诊断上的研究，如耐受性（负荷）试验和（或）小肠枯膜的分析（省略部分不予补充） 。 |
| 7. | After the pbd i and pbd ii gene ligated with the expression vector pinpoint ? a - 3 , the recombinated plasmid ppd - 1 and ppd - 2 was transformed into jm109 strains , 4 positive clones were screened by restriction enzyme analysis , dna sequencing showed that two out of 4 positive clones inserted sequence of the constructed plasmid , which was the same as that of pbd - i and pbd - ii gene respectively , and its reading frame was correct , thus its could be used to express fusion protein
将pbd - 、 pbd -基因与表达载体pinpoint ~ ( tm ) xa - 3连结后获得的重组质粒ppd - 1 、 ppd - 2转化于大肠杆菌jm109中。经抽提质粒、酶切分析及pcr扩增，分别筛选到4个阳性克隆，将其中二个阳性克隆由测序分析，证实1个含pbd i基因片断， 1个含pbd 11基因片断，且阅读框正确，可用于融合蛋白表达。 |
| 8. | Abstract : the polymorphism of angiotensinogen gene at position 174 was studied in 90 cases of essential hypertension patients and 109 controls by pcr , restriction enzyme analysis and electrophoresis methods . the results showed the distribution of genetypes in hypertension group was significantly different from that of controls group . this suggested there is a correlation between the variant of agt174 and hypertension
摘要本文采用pcr 、限制性酶切和电泳分型等方法,分别对90例原发性高血压患者和109例正常人血管紧张素原基因多态位点agt174进行了检测,结果表明,高血压组中三种基因型的分布与对照组显著不同,提高该位点变异与原发性高血压的发生相关。 |
| 9. | Acetylornithine deacetylase is the key enzyme of producting l - methionine . we mainly do research work on the construction of acetylornithine deacetylase gene - engineering strain and characteristic of proteinase . in order to get high expression deacetylase strain , we obtain the gene by pcr arge gene . the product ( 2800bp ) was cloned into puc19 plasmid and confirmed with blue / white dot screening > restriction enzyme analysis and pcr . then taking the nucleotide sequencing compared with the sequence at blast of u . s . a . we constructed a high expression of gene - engineering strain - pxj 128 which containing the arge gene on the high expressing system of pxji18 with activity of acetylornithine deacetylase above 20000u / g
为了获得高效表达的脱乙酰鸟氨酸酶工程菌株，在工程菌技术改造及其固定化研究做了进一步的研究和探讨。我们采用基因工程技术，通过pcr技术扩增出了酰化酶关键酶基因?脱乙酰鸟氨酸酶基因arge ，将其克隆到puc19载体中，经酶切鉴定、 pcr鉴定筛选出重组阳性质粒，并测序鉴定，通过美国blast程序进行了基因数据库相似性比较分析。 |
| 10. | Ii ) two fragments ( about 240bp and 130bp ) were amplified from human keratinocytes total rna by rt - pcr . the recombinant pm - hpabl and pm - hpabs plasmids were constructed by inserting 240bp and 130bp pcr products into pmd 18 - t vector , respectively . the recombinants were identified by restriction enzyme analysis and dna sequencing , iii ) two orfs ( > 100bp ) were found in the insert sequence of pm - hpabl
应用smartpcr试剂盒和简并引物从人皮肤角质形成细胞cdna中扩增到长分别为24obp和13obp左右的2种片段，将它们插入pmd18一t载体，用酶切法初步筛选阳性重组子pm . hrabl和pm一hrabs ，对阳性重组子进一步作测序鉴定。 |
